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Introduction to Single Cell Sequencing Analysis (7): PHATE or Fate
In single-cell data analysis, the most critical step can be said to be the result of cell clustering; and cell clustering involves the design of many mathematical models, whether it is high-dimensional data conversion (dimensionality reduction), noise removal (denoising) and extraction Core information, etc., will affect the final cell clustering results, and therefore there are continuous methods to innovate in order to truly show the clustering distribution of cells. PHATE (Potential of Heat diffusion for Affinity-based Transition Embedding) [1] is a new way of dimensionality reduction. Before introducing this new method, the editor would like to review the past list with you The common dimensionality reduction methods of cell analysis…
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Mouse Ab on mouse tissue
Why use mouse stain kit (article number: 414321F)? Mouse-derived antibodies have a high background in mouse tissues. Reasons: In IHC experiments, the endogenous mouse IgGinmouse tissues will be recognized by the anti-mouse secondary antibody, causing the signal of the target protein to be easily interfered by the high background value, causing interpretation Not easy. Advantages of Fab’fragmet secondary antibody ⭐Can effectively reduce non-specific signals caused by binding to cell surface Fc receptor ⭐Small molecular structure, easier to bind to the target protein in immune tissue experiments
Laboratory Research Protocols
